Hyperspectral Imaging for Assessing Quality and Safety of Meat

Hyperspectral imaging (HSI) technology is a novel nondestructive method and has found various applications in the agricultural and food industry. In this chapter, the employment of HSI for meat quality assessment and safety control was summarized. The quality attributes include sensory attributes (color and marbling), chemical attributes (moisture, protein, intramuscular fat, and fatty acids), and technological attributes (pH, tenderness, and water holding capacity (WHC)). The safety attributes mainly include bacterial contamination and freshness determination. The spectral method is described in terms of the basic working principle, fundamental configurations, analysis period, and applications in meat assessment. In addition, the advantages, disadvantages, and problems to be tackled facing the HSI are also discussed. The current studies have demonstrated that HSI technology can be a potential tool to replace the traditional method for online and simultaneous evaluation of multiple quality and safety attributes of meat

Optimization of hole spacing for cut-top blasting based on new hole-sealing technology

In order to solve the problem of stress concentration on the roof of the mining trench, reduce the risk of sudden collapse of the roof overburden and disturbance, improve the control effect of the surrounding rock of the roof cutting and retaining roadway, and reduce the construction cost of mining tunnels, a new type of pouch sealing technology has been developed. By using on-site testing methods, the optimal sealing material ratio was optimized, and the crack propagation law and roof cutting effect of the 11503 W working face in Zhaizhen Coal Mine, Shandong Province were studied under hole spacing of 0.7, 1.0, and 1.1 meters and different sealing methods. The results show that using 1.5 m single pouch sealing technology in the blasting test, when the water cement ratio of the sealing material is 1:1, the required sealing strength and sealing temperature can be achieved, and there will be no punching phenomenon. When the spacing between holes is 1 m, the blasting effect is optimal, with a single hole effectively reaching a cutting seam length of about 0.5 m. There are obvious through cracks in the cave, with a total length of about 7 meters. After using the new pouch sealing technology for blasting, the displacement and bottom drum volume on both sides of the tunnel are lower than those of the traditional yellow mud sealing method, and the bottom drum volume is reduced by 37% and 53%, respectively. Based on comprehensive theoretical analysis and on-site experiments, the optimal hole spacing is determined to be 1 m, and the pouch sealing effect is good

Adverse events associated with molnupiravir: a real-world disproportionality analysis in food and drug administration adverse event reporting system

Molnupiravir, an urgently approved drug during the Coronavirus Disease 2019 (COVID-19) pandemic, serves as the basis for our study, which relies on the Food and Drug Administration Adverse Event Reporting System (FAERS). The objective is to extract adverse event (AE) signals associated with molnupiravir from the FAERS database, thereby providing a reference for post-marketing monitoring of adverse events. Specifically, we extracted individual case safety reports (ICSRs) from the database, focusing on cases with COVID-19 indications and molnupiravir identified as the primary suspect drug. Descriptive analysis of the extracted data was performed, followed by four disproportionality analyses using the reporting odds ratio (ROR) method. These analyses were conducted across four levels, encompassing overall data, reports by health professionals, as well as age and gender differentiations, ensuring the robustness of the analysis results. In total, 116,576 ICSRs with COVID-19 indications and 2,285 ICSRs with molnupiravir as the primary suspect were extracted. Notably, after excluding cases with unknown age or gender, a higher proportion of molnupiravir-related ICSRs were observed among individuals aged 65 years and older (70.07%) and women (54.06%). The most frequently reported adverse events and AE signals were associated with gastrointestinal disorders, as well as skin and subcutaneous tissue disorders. Moreover, individuals aged 65 years and older exhibited a higher risk of cardiac disorders, hepatobiliary disorders, renal and urinary disorders, and vascular disorders. In conclusion, this study found molnupiravir demonstrated a lower risk of serious adverse events compared to other RNA antiviral drugs like remdesivir in patients under 65 years old. However, close monitoring of its safety is still necessary for elderly patients aged 65 years and above. Further studies are warranted to continuously assess the safety profile of molnupiravir as its usage increases, especially in high risk populations

Targeting microRNAs to Regulate the Integrity of the Blood–Brain Barrier

The blood–brain barrier (BBB) is a highly specialized neurovascular unit that protects the brain from potentially harmful substances. In addition, the BBB also engages in the exchange of essential nutrients between the vasculature and brain parenchyma, which is critical for brain homeostasis. Brain diseases, including neurological disorders and cerebrovascular diseases, are often associated with disrupted BBB integrity, evidenced by increased permeability. Therefore, defining the mechanisms underlying the regulation of BBB integrity is crucial for the development of novel therapeutics targeting brain diseases. MicroRNAs (miRNA), a type of small non-coding RNAs, are emerging as an important regulator of BBB integrity. Here we review recent developments related to the role of miRNAs in regulating BBB integrity

Clinical Application of Exosome Components

Exosomes belong to a subpopulation of EVs that carry different functional molecular cargoes, including proteins, nucleic acids, metabolites, and lipids. Notably, evidence has demonstrated that exosomes participate in bidirectional cell–cell communication and act as critical molecular vehicles in regulating numerous physiological and pathological processes. Since the specific contents within exosomes carry the information from their cells of origin, this property permits exosomes to act as valuable biomarkers. This chapter summarizes the potential use of exosome components in diagnosing, prognosis, or monitoring and treating multiple cancers and other non-neoplastic diseases. We also discuss the deficiency of basic applications, including the limitations of research methods and different research institutions and the differences generated by specimen sources. Thus, a better understanding of the problem of exosome detection may pave the way to promising exosome-based clinical applications

The Pichia pastoris transmembrane protein GT1 is a glycerol transporter and relieves the repression of glycerol on AOX1 expression

Promoter of alcohol oxidase I (PAOX1) is the most efficient promoter involved in the regulation of recombinant protein expression in Pichia pastoris (P. pastoris). PAOX1 is tightly repressed by the presence of glycerol in the culture medium; thus, glycerol must be exhausted before methanol can be taken up by P. pastoris and the expression of the heterologous protein can be induced. In this study, a candidate glycerol transporter (GT1, GeneID: 8197545) was identified, and its role was confirmed by further studies (e.g. bioinformatics analysis, heterologous complementation in Schizosaccharomyces pombe (S. pombe)). When GT1 is co-expressed with enhanced green fluorescent protein (EGFP), it localizes to the membrane and S. pombe carrying gt1 but not the wild-type strain can grow on medium containing glycerol as the sole carbon source. The present study is the first to report that AOX1 in the X-33gt1 mutant can achieve constitutive expression in medium containing glycerol; thus, knocking down gt1 can eliminate the glycerol repression of PAOX1 in P. pastoris. These results suggest that the glycerol transporter may participate in the process of PAOX1 inhibition in glycerol medium

Summer extreme consecutive dry days over Northeast China in the changing climate: Observed features and projected future changes based on CESM-LE

Northeast China (NEC) is a major crop base in East Asia, and summer drought is one of the climate extremes that significantly influences NEC agricultural production. Therefore, understanding the response of NEC summer drought to global warming is of significance. In this study, based on observation and large-ensemble simulations of the Community Earth System Model (CESM-LE), the variabilities in summer extreme consecutive dry days (CDDs) over NEC are investigated in the present and future climate. In the observation, the NEC summer extreme CDDs showed an increasing trend during the past half century and experienced a significant interdecadal change around the middle 1990s, which is mainly due to the change in the anticyclone over Lake Baikal-Northeast Asia. The anticyclone-related anomalous downward motion and moisture divergence provided favorable conditions for increased summer CDDs over NEC. The CESM-LE multimember ensemble (MME) simulation could reproduce the change in NEC summer extreme CDDs and its related atmospheric circulations, indicating that the observed change in NEC summer extreme CDDs could be largely contributed by anthropogenic forcing. In the future warmer climate, the NEC summer extreme CDDs are projected to show interdecadal variability, which increase by approximately 6.7% in the early 21st century (2020–2030), then decrease by approximately 0.3% in the middle to late 21st century (2040–2080), and further increase by approximately 2.1% in the late 21st century (2085–2100). In addition, the projected changes in the anticyclone over Lake Baikal-Northeast Asia show a similar feature to that of the NEC summer extreme CDDs, which might further provide some confidence in the projection of the NEC summer extreme CDDs due to the physical connection between CDDs and anticyclone in the future

Effect of Nano-clay Filler on the Thermal Breakdown Mechanism and Lifespan of Polypropylene Film under AC Fields

The wide application of nanocomposites in the insulation system has greatly contributed to the performance improvement of power equipment. However, nano fillers are not omnipotent for improving the properties of composite dielectrics. In some situations, nano-modified materials are in fact a compromise of improving some performance features while sacrificing others. In this work, the breakdown characteristics and time-to-failure of polypropylene film with nano-clay fillers have been evaluated under combined thermal stress and AC electric fields. Experiments on plain polypropylene (PP) samples have also been carried out under the same test conditions as control. Test results indicated that the time-to-failure of the samples with nano-clay filler was shorter than those without nano filler, which is different from the previous experience. SEM and EDS analyses were conducted to study how the failure mechanism had taken place in both plain polypropylene and the nano-clay filled polypropylene. The failure phenomenon in these materials can be explained by molecular thermodynamics. The main reason for the premature thermal breakdown of PP nanocomposite is essentially due to the weak coupling between nano-clay filler and polymer matrix. Finally, suggestions are proposed for nano modification methods and lifespan prediction models of composite dielectrics